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KMID : 0878220070190010160
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Journal of Sasang Constitutional Medicine
2007 Volume.19 No. 1 p.160 ~ p.170
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An Experimental Study on the Thrombolytic Activities of Holotrichia Extracts
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Kang Min-Seok
Kim Shin
Cho Jae-Seung
Kim Hyo-Soo
Kim Il-Hwna
Park Hye-Sun
Seo Eun-Hee
Yim Chi-Hye
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Abstract
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1.Objectives This study was performed to find the activities and characteristics of purified thrombolytic enzymes from Holotrichia extracts.
2.Methods In the first time, a coarse enzyme fluid was made by using the freedried Holotrichia extracts. After manufacturing total soluble proteins and purifing enzymes, it was evauluated the activities and characteristics of this enzyme’s dissolving capability to fibrin and thrombus. This study was taken using azocasein assay, fibrin-plate method, native-PAGE and fibrin zymography.
3.Results A soluble proteins were efficiently extracted form freezedried Holotrichia extracts. And, this purified enzyme had a ten times fibrinolytic capability compare with ustulation Holotrichia sample. In native PAGE and fibrin zymography, Holotrichia extracts showed the respectable fibrinolytic activity. Also, It had higher thrombolytic activities compared with general thrombolytic enzyme ’plasmin’. In experiment of various protease inhibitors of the purified enzyme from Holotrichia extracts on the azocaseinolytic activity, the enzyme was strongly inhibited by EDTA EGTA, and weakly by APMSF PMSF TPCK.
4.Conclusion Holotrichia extracts has the thrombolytic activities, and it will operate directly th fibrin-clot and thrombus.
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KEYWORD
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Holotrichia, Thrombolysis, Fibrin, Thrombus, Enzyme, Taeum-in
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